Composite

Part:BBa_K2760032:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-12)


Signal peptide negative control characterization construct in L. rhamnosus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
    Illegal AgeI site found at 836
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    • Optimized for L. rhamnosus


Source

This plasmid expression vector has a spaCBA promoter, it contains a specific RBS for L. rhamnosus GG, M-cherry ( BBa_K2760029) as the fluorescent reporter with a His-Tag (6 histidines) attached to it; and the terminator 667.

References